Recombination-deficient mutant of Streptococcus faecalis
نویسندگان
چکیده
منابع مشابه
Recombination-deficient mutant of Streptococcus faecalis.
An ultraviolet radiation-sensitive derivative of Streptococcus faecalis strain JH2-2 was isolated and found to be deficient in recombination, using a plasmid-plasmid recombination system. The strain was sensitive to chemical agents which interact with deoxyribonucleic acid and also underwent deoxyribonucleic acid degradation after ultraviolet irradiation. Thus, the mutant has properties similar...
متن کاملIsolation of a Recombination-Deficient Mutant of Streptococcus lactis ML3t
A recombination-deficient mutant of Streptococcus lactis ML3 designated MMS36 was isolated on the basis of its sensitivity to methyl methanesulfonate. This mutant also displayed sensitivity to UV irradiation. The inability of MMS36 to mediate homologous recombination was demonstrated by transduction of plasmid-linked lactose fermenting ability but not chromosomally mediated streptomycin resista...
متن کاملIsolation of a recombination-deficient mutant of Streptococcus lactis ML3.
A recombination-deficient mutant of Streptococcus lactis ML3 designated MMS36 was isolated on the basis of its sensitivity to methyl methanesulfonate. This mutant also displayed sensitivity to UV irradiation. The inability of MMS36 to mediate homologous recombination was demonstrated by transduction of plasmid-linked lactose fermenting ability but not chromosomally mediated streptomycin resista...
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A UV-sensitive derivative was obtained from Streptococcus sanguis Challis. The organism could be transformed with a number of small streptococcal plasmids at frequencies equal to, or 1 logarithm below, the transformation frequencies for the parent organism. However, transformation with chromosomal DNA was greatly impaired in the UV-sensitive derivative.
متن کاملProperties of a dicarboxylic amino acid transport-deficient mutant of Streptococcus faecalis.
A mutant of Sfrepfococcus faecalis has been isolated which has a ZO-fold higher growth requirement for L-glutamic acid than the parent strain. The ability to utilize L-glutamine for growth is not affected. The mutant also has a greater dependence than the parent strain on an external supply of vitamin B6. The rate of glutamic acid transport at low extracellular concentrations (0.05 mu) was grea...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1980
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.143.2.966-970.1980